The isolation and regeneration of potato (Solanum tuberosum L) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid . The Isolation of regeneratiom of potato (Solanum tuberosum L.) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid. One of new technologies in order to create hybrid plant from different species is protoplast fusion. This technique involves protoplast isolation, purification.

Author: Fauhn Mikam
Country: Madagascar
Language: English (Spanish)
Genre: Love
Published (Last): 6 December 2006
Pages: 379
PDF File Size: 18.12 Mb
ePub File Size: 15.26 Mb
ISBN: 574-2-63595-756-5
Downloads: 98362
Price: Free* [*Free Regsitration Required]
Uploader: Vuramar

Protoplast were cultured on the medium of Ikeda and Uchimia. Indonesian, English Full-text formats available: Funding for sustainable Open Access incl. Publication Year Publication Year.


Close Go to Workspace. The results showed that there was effect of treatment for all variables observed.

Our members Our publisher members Our sponsors Our volunteers. They were incibated in full darkness at 27 Celcius centigrade by constant shaking at 50 rpm orbitor shaker. Indonesia Language of fulltext: Regeneration of cell wall observed progessively in mother protoplast from tobacco, cowpea A27, E1, and H6 and fused protoplast of soybean with tobacco, tobacco with cowpea C4, E1, and H6soybean with cowpea C4 isolawi between cowpea C4 and cowpea E1.


Results 1 – 1 of 1. The first factor was concentrations of sucrose which consists of three levels namely 0.

Isolasi dan Regenerasi Protoplas dari Mesofil daun Kentang (Solanum tuberosum L) Dihaploid

Journal on Agriculture Science. The second factor was incubation time which consists of four levels namely 2 hours, 4 hours, 6 hours and 8 hours. Looks like Javascript is disabled on your browser. Fusion was induced with PEG solution according to Uchimia and fused protoplasts were collected by centrifugation of g. The factorial experiment consisted of two factors that was arranged by completely randomized design CRD with ten replications.

Leaf protoplast were releasaed in h, soybean and tobacco callus protoplast in h, and cowpeas callus protoplast in h of incubation. Cell wall generation was observed.

Prosiding Seminar Nasional Biologi

Javascript must be enabled for narrowing. Please provide a name for this query: Isolasi dan fusi protoplas dari kedelai, buncis, dan tembakau. Isolation of protoplast from soybean, cowpea, and tobacco and their fusion. Reference Number Isolaso Number.

For soybean leaf, the medium was enriched with driselase and pectolyase Y Agriculture General Country of publisher: Standard Search Advanced Search. Get permanent URL for this record.


Protoplast were collected by centrifugation of g and a thin layer of the suspension was irradiated with ultraviolet light. The obyectives of the research was to investigate the effect of combination treatment of sucrose concentrations and isoalsi time on protoplast isolation of the leaves mesophyll orchid of Paraphalaenopsis layockii.

Callus wich has been two times resubcultured was suspended in the digestive medium without driselase, CaCl2, and MES and incubated isolaei lowlight intensity by constant shaking at rpm in reciprocal water shaker at 30 celcius centigrade.

Journal Homepage Journal Title: On both medium leaf protoplast, irradiated protoplasts and their fused do not regenerate cell wall and all cultured died out within four weeks incubation. The highest protoplast density and number usolasi viable protoplasts achieved in the combined treatment of 0. Data were analyzed using Kruskal-Wallis test followed by Mann-Whitney test.

Author: admin